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This Conference is FREE, advance registration is essential as places are limited to 90, advance registration is requested.

Programme

09.55 Introduction and Welcome
Laurence Devereux, Xmark Media, on behalf of the programme committee
Session 1: Photonics in Medicine
Chair: Dr Alastair McInroy
10.05 Recent Advances in Photodermatology and Photodynamic Therapy
Dr Ewan Eadie, NHS Tayside
Photodermatology is the study of light interactions with the skin and can be broadly separated into two categories, therapy and diagnostics. Light has been used as a therapy for skin diseases since ancient times, with modern therapy in the form of ultraviolet emitting fluorescent lamps being utilised since the 1980s. Technological advances in Photodermatology, particularly in light source development, have been poor mainly due to a lack of commercial incentive. However recent advances in light emitting diodes (LED) and digital mirror devices are attempting to address the current fluorescent lamp limitations and increase uptake of ultraviolet phototherapy. Photodynamic therapy (PDT), a treatment for cancer that photoactivates a photosensitiser in the presence of oxygen to destroy diseased tissue, is similarly experiencing marked expansion of research activity. Although always popular in Photodermatology, PDT is being driven forwards by new photosensitising agents for cancer treatment and as a potentially important alternative to drug treatment in antimicrobial resistance.

There is also prominent research activity in light based photodiagnostic non-invasive imaging techniques such as optical coherence tomography and Raman spectroscopy, with interesting preliminary clinical data in skin diseases. These techniques are yet to translate into routine clinical use but it is an exciting time to be involved with photonics and skin.
10.30

“Lighting up the Lung”- Beginning the journey to understand disease before we can diagnose or treat it 
Dr Kev Dhaliwal, University of Edinburgh

The talk will introduce the threat of antimicrobial resistance and the global crisis that faces us in modern healthcare. The talk will then introduce an EPSRC Project between 3 universities – Edinburgh , Heriott Watt and Bath which is pushing the boundaries of biophotonic sensing and imaging to begin understanding infection in humans so we can better diagnose, treat and manage infection.

10.55 Imaging of the human retina
Dr Jano Van Hemert, Optos Plc
Optos plc (a Nikon company) has the vision to be The Retina Company. We review different imaging modalities of Optos ophthalmoscopes and the properties and sources of light they depend on. All of these products are used to identify and quantify structural damage in the retina. Once structural damage is present little can be done to reverse this. Therefore we should identify pathologies in the retina as early as possible. Loss of function can occur prior to structural damage. We introduce a novel device, now in a clinical trial, that aims to measure objectively functional loss of the retina. 
11.20 break in the exhibition hall
Session 2: Advances in Optical Manipulation and Microscopy
Chair: Dr Lynn Paterson
11.45 Holographic micro-endoscopy through multimode waveguides
Dr Tomas Cizmar, University of Dundee

The turbid nature of refractive index distribution within living tissues introduces severe aberrations to light propagation thereby severely compromising image reconstruction using currently available non-invasive techniques. Numerous approaches of endoscopy, based mainly on fibre bundles or GRIN-lenses, allow imaging within extended depths of turbid tissues, however their footprint causes profound mechanical damage to all overlying regions and their imaging performance is very limited. Progress in the domain of complex photonics enabled a new generation of minimally invasive, high-resolution endoscopes by substitution of the Fourier-based image relays with a holographic control of light propagating through apparently randomizing multimode optical waveguides. This form of endo-microscopy became recently a very attractive way to provide minimally invasive insight into hard-to-access locations within living objects.
I will review our fundamental and technological progression in this domain and introduce several applications of this concept in bio-medically relevant environments.  I will present isotropic volumetric imaging based on advanced modes of light-sheet microscopy: by taking advantage of the cylindrical symmetry of the fibre. Further, I will demonstrate the first utilization of multimode fibers for imaging in living organism and present a new fibre-based geometry for deep tissue imaging in brain tissue of a living animal model.
Lastly I will show the development and exploitation of highly specialized fiber probes for numerous advanced bio-photonics applications including high-resolution imaging and optical manipulation.

KEYNOTE
12.10 Optical manipulation and biomedical applications of HOT nanoparticles
Professor Lene Broeng Oddershede, NBI, University of Copenhagen
  A tightly focused laser beam can trap and manipulate individual metallic nanoparticles both in liquid and in air. Not only the position, but also the orientation of a single nanoparticle can be controlled and such precise optical control of metallic nanoparticles has huge potential, e.g., for nano-architectural purposes or for amplifying spectroscopic signals. Due to their plasmonic properties, metallic nanoparticles will absorb part of the incident light and release the energy as heat into their local surroundings. The heating associated with resonant irradiation of metallic nanoparticles can be extreme. We developed a membrane-based assay to directly quantify the absorbance and temperature profile of an individual irradiated metallic nanoparticle and show how the temperature profile depends on laser power and particle size, shape, orientation and composition. Also, we demonstrate how to utilize laser induced heating of metallic nanoparticles in a controlled manner to fully fuse two selected living cells or giant unilamellar vesicles, a realization of single cell drug delivery. Hot metallic nanoparticles also have huge potential for cancer therapy, where we demonstrate how laser irradiated metallic nanoparticles can be used for photothermal treatment of tumors in living mice.
12.50 Intensity based three-dimensional super-resolution imaging
Dr Sebastian Van de Linde, University of Strathclyde
We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.
13.15 lunch break - opportunity to review the Exhibition
14.15 Poster Session in the exhibition hall
Session 3: Optics and Cells
Chair: Professor Rory Duncan
15.00 The Latest Optoelectronic Gadgets for Cells – New Biophotonic Tools for Cell Biology and Beyond
Professor Malte Gather, University of St. Andrews
I will present our recent work on new micro- and nano-photonic devices for studying cells and their behaviour. Our lab is probably most well-known for the invention of the biological laser, which recently made it into the Guinness World Records. By inserting microlasers into live cells we are able to tag and track individual cells in large cell populations over extended periods of time and are currently developing this technology to follow migration of cancer and immune cells.

Beyond these unique lasers, the lab works on functional imaging modalities to study the mechanical properties of single cells at unprecedented resolution. During my talk, I will cover recent work on biolasers and introduce our micro-cavity based photonic sensor platform which allows us to follow the movement and mechanical action of primary cells over much longer times than possible with current state-of-the-art technology. I will also discuss our recent development of an organic LED based platform technology, which achieves optogenetic manipulation of cell behaviour via microscopic versions of the AMOLED displays found in modern smartphones.
15.25 Optimal Fluorophores for Maximising FLIM-FRET Biosensor Capabilities
Dr Kirsty Martin, Beatson Institute for Cancer Research
FRET biosensors have proven very useful tools for studying the activation of specific signalling pathways in living cells. Most biosensors designed to date have been predicated on fluorescent protein pairs that were identified by, and for use in, intensity based measurements, however fluorescence lifetime provides a more reliable measurement of FRET. Both the technology and fluorescent proteins available for FRET have moved on dramatically in the last decade, with lifetime imaging systems have become increasingly accessible and user-friendly, and there is an entire field of biology dedicated to refining and adapting different characteristics of existing and novel fluorescent proteins.  We have exploited this growing pool of fluorescents proteins, identifying long-lifetime green and cyan fluorescent proteins Clover and mTurquoise2 and the dark acceptor sReACh, and have characterised their behaviour in FLIM-FRET conditions with the aim of providing more reliable, reproducible and quantifiable FLIM-FRET data to improve the dynamic range and breadth of application of biosensor technologies.
15.50 Optical manipulation and sensing within femtosecond-laser fabricated devices
Dr Lynn Paterson, Heriot Watt University
  There is a requirement from the life sciences community to rapidly manipulate and detect single cells for the purposes of cell sorting and cell identification. We are addressing this need by creating functional microfluidic devices using the techniques of ultrafast laser inscription (ULI) and selective chemical etching. Buried, three dimensional, microfluidic circuitry has been fabricated in fused silica to enable the handling of small volumes of liquid. Integrated waveguides have been incorporated, using ULI, enabling the controlled optical manipulation of particles in the channel. SERS substrates have been laser-written within the channels and initial results show promise for particle identification. Combining these approaches, we hope to create miniaturized cell sorters and sensors that will find use as point of care devices.
16.15 Closing remarks
16.20 Awarding of Poster Prize
  The Exhibition will remain open until 5pm

 

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PROGRAMME
COMMITTEE

Dr Graeme Whyte (Chair)
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University.

Dr Tom Brown
Reader in Photonics
University of St Andrews

Prof Rory Duncan
Head of Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University

Prof Gail McConnell
Chair of Biophotonics at the Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde

Dr Ally McInroy
Senior Programme Manager, Technology Scotland

Dr Lynn Paterson
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University

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